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This study presents the genetic and molecular characterization of the helices or S-layers of Trichoderma helices, a novel high-temperature induced mutant strain of Trichoderma viride. The principal structural and functional genes were analyzed by heterokaryon complementation, polymerase chain reaction, Southern hybridization, and rDNA-based restriction pattern studies. The mutant strain HSS22 contained a 1.2 Mb continuous chromosome and no evidence of the 7.8 Mb and the 31.7 Mb chromosomes that are known to be present in the wild-type parent. The mutant strain HSS22 had no detectable transcripts, including the Hel1-encoding gene or the Hel3-encoding gene. The wild-type and the mutant strains had identical DNA-DNA hybridization profiles and no cross-hybridization of the mutant strain HSS22 to DNA from a variety of other fungal genera. The amino acid sequence of the putative Hel2-encoding gene of T. viride, amplified from genomic DNA of strain HSS22, showed only minor differences compared to the amino acid sequence of the corresponding gene of the wild-type strain. The mutant strain HSS22 was also unable to grow at 30 degrees C on rich media, except in the presence of Hel1- and Hel3-encoding sequences from a heterokaryon containing the 31.7 Mb chromosome. In the heterokaryon containing the 1.2 Mb chromosome and the partial Hel1-encoding gene, however, the mutant strain HSS22 grew rapidly at 30 degrees C.MUMBAI: Soon, those who wish to donate their kidneys to save the lives of others will have to get certified by a government-appointed doctor. NDA goes for amendments to a law on organ donation so as to include scanning and certification.
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