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Category:2005 video games
Category:Grand Theft Auto
Category:PlayStation 2 games
Category:Video games developed in Canada
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Category:Video games set in the United StatesL-arabinose protects human endothelial cells from apoptosis induced by homocysteine.
Hyperhomocysteinemia (HHcy) is an independent risk factor for thrombotic cardiovascular disease. The pathogenesis of cardiovascular disease has been recognized to be closely related to the balance of pro- and anti-apoptotic proteins. In recent studies, L-arabinose was found to protect vascular endothelial cells from apoptosis in vitro. However, whether L-arabinose can prevent endothelial cell apoptosis induced by HHcy is unclear. In this study, we investigated whether L-arabinose could protect human endothelial cells from apoptosis induced by homocysteine (Hcy). Human endothelial cells (EA.hy926) were incubated with 10-100 microM Hcy in the presence of L-arabinose (1-10mM) for 48h. Apoptosis was determined by flow cytometry and TUNEL assay. Our results show that Hcy increases apoptosis in EA.hy926 cells in a dose- and time-dependent manner, and that L-arabinose has anti-apoptotic effect on endothelial cells incubated with 10-100 microM Hcy. The effect of L-arabinose on Hcy-induced endothelial cell apoptosis was increased with concentration. Moreover, L-arabinose decreases intracellular reactive oxygen species, total glutathione, reduced glutathione, and GSH/GSSG ratio in 10 microM Hcy-treated endothelial cells. These results suggest that L